Table of Contents
What is Peptide Reconstitution?
Reconstitution is the process of dissolving a lyophilized (freeze-dried) peptide powder into a liquid solvent — almost always bacteriostatic water — to create a stable injectable solution. Peptides are shipped and stored as dry powder because the lyophilized form is far more stable and has a significantly longer shelf life than a liquid solution.
The volume of BAC water you add directly determines the concentration of your solution, which in turn affects all subsequent dosage calculations. Use our reconstitution calculator to find the right water amount.
What You Need (Educational Overview)
- Lyophilized peptide vial — labeled with total mass in mg
- Bacteriostatic water — pharmaceutical-grade, 0.9% benzyl alcohol
- Insulin syringe — U-100 for precise measurement
- Alcohol swabs — to sterilize vial tops before use
- Label — to record concentration, date, and peptide name
How Much BAC Water to Use
The amount determines concentration. More water = lower concentration = larger draw volume per dose. Less water = higher concentration = smaller draw. Use our BAC Water Calculator to work backwards from a preferred draw volume.
Concentration (mcg/ml) = (Vial mg × 1000) ÷ BAC Water (ml)
| Vial | BAC Water | Concentration |
|---|---|---|
| 5 mg | 1 ml | 5,000 mcg/ml |
| 5 mg | 2 ml | 2,500 mcg/ml |
| 10 mg | 2 ml | 5,000 mcg/ml |
Step-by-Step Process (Educational)
- Calculate BAC water volume using the reconstitution calculator.
- Wipe both vial tops with alcohol swabs. Allow to air dry.
- Draw calculated BAC water into a syringe.
- Insert the needle at an angle and inject slowly down the inside wall of the vial — not directly onto the powder.
- Gently swirl — never shake — until the powder fully dissolves (30–60 seconds).
- Inspect: solution should be clear and colorless. Discard if cloudy or particulate.
- Label immediately: peptide name, concentration, date reconstituted.
Storage After Reconstitution
General educational guidelines (not medical advice):
- Refrigerator (2–8°C): Most BAC water reconstitutions remain stable for 4–8 weeks.
- Freezer (−20°C): For longer storage, freeze in single-use aliquots. Avoid repeated freeze-thaw.
- Avoid light and shaking. Always handle vials gently.
Always consult the specific peptide's technical data sheet for compound-specific storage conditions.
Common Mistakes (Educational)
- Injecting water directly onto powder — can cause foaming and degradation. Aim for the glass wall.
- Shaking the vial — agitation can break peptide bonds. Swirl gently instead.
- Not labeling — you'll lose track of concentration, making dosage calculations impossible.
- Wrong water volume — always calculate first with the BAC water calculator.